Dithiothreitol (DTT, Cleland’s reagent) for biotechnology 5G

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0281-5G

Description

Synonyms: DL-1,4-Dithiothreitol , DTT , (R*,R*)-1,4-Dimercapto-2,3-butandiol

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Formula: C₄H₁₀O₂S₂
MW: 154.25 g/mol
Boiling Pt: ∼125 °C (1013 hPa)
Melting Pt: 42 C…43 °C
Flash Pt: >110 °C
Storage Temperature: Refrigerator
MDL Number: MFCD00004877
CAS Number: 3483-12-3
EINECS: 222-468-7
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