DNA Oligonucleotide Synthesis 50 nmol scale

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EO-6400-50n

Description

Gel Purification

All Gene Link oligos shorter than 40 mer do not require further purification if the applicaton is for PCR or sequencing. 20 mer oligo synthesized at a coupling efficiency of 99.5% will contain ~90% full length 20 mer and a mixture of truncated sequences of ~10%. As the length of the oligo increases, even at a coupling efficiency of 99.5%, the yield of the full length oligo is reducing. A 60 mer crude product will contain ~75% full-length oligo and similarly a 100 mer will contain ~60%. Purification is strongly recommended for oligos longer than 50 mer.

Oligo Size, Scale of Synthesis & Purification Guidelines
Product 50 nmol scale 200 nmol scale 1 umol scale 2 umol scale 10 umol scale 15 umol scale
Maximum Size 49 mer 99 mer 180 mer 250 mer 250 mer 250 mer
Purification Grade PAGE or RPC up to 35mer PAGE PAGE PAGE PAGE PAGE
All Gene Link oligos shorter than 40 mer usually do not require any further purification if the application is for PCR or sequencing.
Purification (price per DNA oligo)
Product 50 nmol scale 200 nmol scale 1 umol scale 2 umol scale 10 umol scale 15 umol scale
Catalog No. (PAGE) 26-6400-77 26-6400-77 26-6400-55 26-6400-88 26-6400-44 26-6400-99
Gel Purification (PAGE) 75.00 75.00 150.00 280.00 1500.00 1800.00
Catalog No. (RPC) 26-6400-11 26-6400-11 26-6400-22 26-6400-23 26-6400-33 26-6400-34
Reverse Phase Purification (RPC) 30.00 30.00 90.00 170.00 750.00 900.00
Scale of Synthesis & Yield of Unmodified DNA Oligos
Oligo Scale of Synthesis and Typical Yield of Unmodified DNA Oligos*
Crude Desalted RPC Purified** Gel Purified
20 mer oligo
Typical Yield
30 mer oligo
Typical Yield
50 mer oligo
Typical Yield
Scale A260 Units nmols mg A260 Units nmols mg A260 Units nmols mg
50 nmol 8-10 30+ 0.2-0.3 4-5 12+ 0.1-0.16 NR* [1-2] NR* [2-4] NR* [0.03-0.06]
200 nmol 20-25 80+ 0.6-0.8 8-12 24+ 0.26-0.4 4-6 8+ 0.13-0.2
umol 100-120 400+ 3-4 40-50 90+ 1.3-1.6 20-25 40+ 0.6-0.8
umol 200-240 800+ 6-8 80-100 180+ 2.5-3.1 40-50 80+ 1.2-1.5
10 umol ~1000 ~4000 ~30 ~400 ~900 ~10 ~200 ~400 ~6
15 umol ~1500 ~6000 ~45 ~600 ~1350 ~15 ~300 ~600 ~9
Purity & Yield Purity is greater than 80%

depending on oligo sequence and structure. Refer to coupling efficiency table for oligo length dependent purity and yield.

No further purification required for PCR and sequencing applications.

Gel purification recommended for oligos above 50 mer and all applications involving cloning and mutagenesis.

**RPC is reverse phase purification using a cartridge; a substitue for HPLC.

Purity 85% to 95%

depending on oligo sequence and structure

Yield and Purity will be lower for sequences with high GC content

Not recommended for oligos longer than 35 mer.

**RPC is reverse phase purification using a cartridge; a substitue for HPLC.

Purity 98% to ~100%

depending on oligo sequence and structure

Yield will gradually decrease as length of oligo increases. Palindromes, hairpins and high GC content oligos and oligos containing stretches of 3 or more G’s induce strong secondary structure and base stacking thus decreasing purity and yield.

*NR Not Recommended

*a. Typical yield stated in the table is for unmodified random sequence oligos. Reduction in yield is observed with high GC content oligos and those forming strong secondary structures.

b. Reduced yield is expected with modified oligos. The reduction percentage varies with modification type and number of sites. Typical reduction is 10%-20% per modified site.

c. Yield of 33µg/A260 unit for DNA oligos is calculated for an ~equimolar base composition. Long stretches of a single base or homopolymers will have variable yields. Example for homopolymeric 20 mer: dA(20) = ~25ug/A260 Unit; dC(20) = ~40ug/A260 Unit; dG(20) = ~32ug/A260 Unit and dT(20) = ~37 ug/A260 Unit

 

Guaranteed delivery in less than 10 working days from the USA to the UAE.

Note: Add shipping costs for less than 1000 bases.

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