bioWORLD’s high quality Gram Stain Kit contains ample amounts of Crystal Violet, Iodine, Decolorizing solution, and Safranin-O Counterstain for a complete stain. Easily differentiate between Gram-positive and Gram-negative bacteria with this simple procedure.
For best results, add 5 drops of Crystal Violet to a small amount of culture fixed to a microscope slide. Rinse off with water after 60 seconds, then add 5 drops of Iodine solution. After 60 seconds, rinse again with water. Add Decolorizing solution just until the solvent runs clear, then add 5 drops of Safranin-O and rinse with water after 60 seconds.
These quality kit solutions may also be purchased individually. Crystal Violet, Decolorizer, Safranin-O Counterstain. Check out our line of Microbiology Grade Stains and Dyes for a number of staining applications.