HOT FIREPol® GC Master Mix

د.إ 417.10
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04-33-00115

Description

Hot-start PCR mix for GC-rich templates

  • excellent amplification with templates up to 79% GC content
  • suitable for templates up to 5 kb
  • suitable for fragment analysis
  • 1 ml | 250 rxn

Description

HOT FIREPol® GC Master Mix has been developed for working with difficult GC-rich templates and DNA secondary structures. It shows excellent amplification with templates up to 79% GC content

The master mix contains hot-start Taq polymerase HOT FIREPol®, MgCl2, dNTPs, and a special buffer for high yield GC-rich amplification.

Extra vials of 100% DMSO and 25 mM MgCl2 enable flexibility in reaction optimization.

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min.

Ready to load: no

Fidelity: 1 x Taq

Cloning Type: T/A cloning

Amplicon Size: up to 5 kb

Difficult templates: robust on GC-rich templates

Mix Composition:

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start

5x HOT FIREPol® GC Buffer with 7.5 mM MgCl21x PCR solution –1.5 mM MgCl2

dNTPs: dATP, dCTP, dGTP and dTTP

Bovine serum albumin (BSA) to enhance PCR reaction

In separate vials:

100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.

25 mM MgClfor further optimization

Item Pcs. Vial size
HOT FIREPol® GC Master Mix 1 1 ml | 250 rxn
25 mM MgCl2 1 0.5 ml
DMSO 100% 1 0.5 ml

SOLIS BIODYNE

Solis BioDyne has been developing and producing life science reagents since 1995, having become one of the leading reagent suppliers in Europe today. High standards for production and service have made Solis BioDyne a trusted trademark worldwide. Solis BioDyne DNA polymerases, PCR Master Mixes, qPCR Mixes and reverse transcription reagents are used by a quickly growing number of customers across the globe, including top research institutes and biotech-companies. Solis BioDyne has partners in both private and state sectors, with cooperation projects ranging from OEM production to scientific research.
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