SOLIScript® 1-step SolisGreen® Kit

د.إ 3,295.10
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Description

Highly sensitive dye-based one-step RT-qPCR

SOLIScript® 1-step SolisGreen® Kit is an optimized solution for highly
sensitive real-time one-step RT-PCR (RT-qPCR) using intercalating
dye detection via the SYBR®/FAM fluorescence channel.

  • cDNA synthesis and dye-based qPCR in one tube
  • high specificity, sensitivity and yield
  • reaction set-up and shippment without ice
  • consistent results with low RNA input
  • RNase inhibitor included in kit
  • 250 rxn | 250 x 20 μl rxn

Description

SOLIScript® 1-step SolisGreen® Kit is optimized for highly sensitive and accurate quantification of RNA targets by real-time one-step RT-PCR (RT-qPCR) using dsDNA-intercalating dye detection via the SYBR®/FAM fluorescence channel.

Compatible with ROX-independent and ROX-dependent qPCR cyclers (excluding high-ROX platforms).

The Kit comes in 3 tubes (including water) and contains all the components necessary (except RNA template and primers) to perform cDNA synthesis and qPCR in a single tube.

Applications

  • Gene expression analysis and absolute quantification
  • Pathogen detection and quantification
  • Low-copy gene detection
  • Gene knockdown validation
  • miRNA profiling and quantification
  • GMO characterization

 

Properties

Sample type: RNA

Product format: Reagent kit

No of tubes: three tubes

Detection type: Intercalating dye-based

Reference dye: reference dye based on ROX

Compatible qPCR cyclers: The Kit is compatible with ROX-independent and ROX-dependent qPCR platforms (excluding high-ROX machines, such as Applied Biosystems™ 5700, 7000, 7700, 7900HT, StepOne™, StepOnePlus™).  Check Your cycler!

Kit Components

40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor

5x One-step SolisGreen® Mix: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl2 (1x RT-qPCR solution – 2.5 mM MgCl2), SolisGreen® dsDNA-intercalating dye, passive reference dye based on ROX dye

SOLIS BIODYNE

Solis BioDyne has been developing and producing life science reagents since 1995, having become one of the leading reagent suppliers in Europe today. High standards for production and service have made Solis BioDyne a trusted trademark worldwide. Solis BioDyne DNA polymerases, PCR Master Mixes, qPCR Mixes and reverse transcription reagents are used by a quickly growing number of customers across the globe, including top research institutes and biotech-companies. Solis BioDyne has partners in both private and state sectors, with cooperation projects ranging from OEM production to scientific research.
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