SOLIScript® Fast 1-step RT-qPCR Mix with UNG

د.إ 1,752.10
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08-87-00200

Description

Convenient single vial format and sensitive 5-plex amplification for demanding one-step RT-qPCR experiments

  • 4x concentrated one-step RT-qPCR reagent for RNA detection
  • Convenient reaction setup with a single vial format
  • Amplify up to 5 targets within one reaction
  • UNG enzyme prevents carryover contamination and false positive results
  • Quick results thanks to fast extension rates of the in silico designed HOT SolisFAST® DNA Polymerase
  • Robust performance in inhibited conditions
  • RNase inhibitor included in the mix
  • 200 rxn | 200 x 20 µl rxn

Description

SOLIScript® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG™ Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.

Applications

  • Detection and quantification of RNA and DNA targets
  • DNA/LNA hydrolysis probe-based assays
  • Suitable for singleplex and multiplex reactions (up to 5 plex)

Properties

Sample type: RNA and DNA
Product format: Reagent kit (nuclease-free water supplied in a separate tube)
Detection type: Probe-based
Reference dye: none
Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, switch off ROX normalization prior to the run. Check your cycler!*

*Version with ROX available upon request.

Reagents

SOLIScript® Fast 1-step RT-qPCR Mix with UNG comprises:

  • 4x SOLIScript® Fast 1-step RT-qPCR Mix with UNG (SOLIScript® Reverse Transcriptase, RiboGripTM RNase Inhibitor, HOT SolisFAST® DNA polymerase, Salini UNGTM Uracil-N-Glycosylase, a unique reaction buffer with dNTPs (including dUTPs), 22.0 mM MgCl2 (1x RT-qPCR solution – 5.5 mM MgCl2) and additives to maximize PCR efficiency).
  • Water, nuclease-free

SOLIS BIODYNE

Solis BioDyne has been developing and producing life science reagents since 1995, having become one of the leading reagent suppliers in Europe today. High standards for production and service have made Solis BioDyne a trusted trademark worldwide. Solis BioDyne DNA polymerases, PCR Master Mixes, qPCR Mixes and reverse transcription reagents are used by a quickly growing number of customers across the globe, including top research institutes and biotech-companies. Solis BioDyne has partners in both private and state sectors, with cooperation projects ranging from OEM production to scientific research.
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