Description

TAE Buffer 10X, Liquid Concentrate.1 L

Product Description TAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 10X concentration and is prepared with 18 megohm water. It has a pH of 8.3 � 0.2. A concentration of 1X contains 0.4M Tris acetate and 0.01M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.