Zymolyase 20T (Lyticase, Yeast Lytic Enzyme); 100 mg

د.إ 2,049.10
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MBS653884

Description

Product Categories/Family for Zymolyase 20T enzyme
Application Notes for Zymolyase 20T enzyme
Protoplast/Spheroplast Preparation, Yeast Cell Fusion, Yeast Cell Transformation

Testing Data of Zymolyase 20T enzyme
The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type
Zymolyase 20T enzyme Testing Data image
Testing Data of Zymolyase 20T enzyme
Cultures from the sin 1 (sln1Δ) strain JF2007, or sin 1 (sln1Δ) ccw 12 (sln1Δ), strain JF2368 transformed with the SLN 1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1 (sln1Δ) ECD), and treated with 1 unit/ml zymolyase (+) where indicated.
NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln 1(sln1Δ)ECD strains
Zymolyase 20T enzyme Testing Data image
Testing Data of Zymolyase 20T enzyme
Source:
Arthrobacter luteus
Zymolyase 20T enzyme Testing Data image

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