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DNase I (RNase-Free) 200 U (100 μl)
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DNase I (RNase-Free) 200 U (100 μl)
| Cat. No. | E091 | ||||||
| Name | DNase I (RNase-Free) | ||||||
| Unit | 200 U (100 μl) | ||||||
| Category | Molecular Biology Enzymes and Kits | ||||||
| Description | DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product is RNase free and can be used in RNA applications.
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| Application | • Removes DNA from protein preparations and RNA samples • Mediates nick translation • Generates random fragments for dideoxy sequencing • Degrade template DNA following in vitro transcription • Mediate DNase I foot-printing |
| Concentration | 2 U/μl |
| Note | One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.
This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C. For non-RNAse-Free DNAse I, see Cat. No. G028. This product is distributed for laboratory research only Caution: Not for diagnostic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E091 |
ABM
Founded in 2004, Applied Biological Materials (abm) Inc. has been catalyzing scientific discoveries in Life Sciences and Drug Development for over a decade. Based in Vancouver, Canada, our agility in developing and commercializing novel research tools and our complete portfolio of unique services have made us an invaluable partner to laboratories and research institutions around the world. Driven by a team of passionate and talented scientists, abm is dedicated to empowering the scientific community with the latest innovations for life sciences research and drug development.
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