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Human Tumor Necrosis Factor Alpha (TNFa) High Sensitive ELISA Kit 96-Strip-Wells

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MBS2707991
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Description

Human Tumor Necrosis Factor Alpha (TNFa) High Sensitive ELISA Kit 96-Strip-Wells

Full Product Name

Human Tumor Necrosis Factor Alpha (TNFa) High Sensitive ELISA Kit

Product Synonym Names
Tumor Necrosis Factor Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of TNFa. No significant cross-reactivity or interference between TNFa and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.56-100pg/mL
Sensitivity
< 0.62pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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