X-Gal-500mg
Description
X-Gal is otherwise known as 5-Bromo-4-chloro-3-indolyl beta-D-Galactopyranoside. When hydrolysis occurs, X-Gal forms a blue pigment. This histochemical substrate for beta-Galactosidase is used in blue/white colony screening to identify bacteria positive for the lacZ gene. Staining with X-Gal has also proven to be effective in identifying grafted cells in tissue transplants.
Molecular Biology Grade X-Gal is soluble in DMF. X-Gal is often accompanied by IPTG for blue/white screening. Most protocols call for a final concentration of 1mM of both reagents.
Properties
Shelf life
5 years
Storage Temperature
-20°C
ECCN #
EAR99
European Com.#
230-640-8
Hazmat Ship
Non-hazardous
Purity
>98%
Appearance color
White
Appearance form
Powder
Molecular Formula
C14H15BrCINO6
Molecular Weight
408.63
Melting point
236-238°C
Solubility (@ RT)
Soluble in DMSO and DMF, (1:1): 50 mg/mL,
Pack Size
500mg
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