5x HOT FIREPol® EvaGreen® qPCR Mix Plus 1 ml | 250 rxn

د.إ 500.10
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08-24-00001

Description

Cost-effective dye-based qPCR Mix with passive reference dye ROX

  • high sensitivity and specificity
  • excellent efficiency
  • reaction set-up and shipment without dry ice
  • cost-effective solution for wide range of applications

Description

Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. In this mix EvaGreen® double-stranded DNA binding dye is used instead of the more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen® dye shows a higher fluorescence level, high sensitivity for detecting low template concentrations, and high stability at room temperature.

HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimized ready-to-use solution for dye-based real-time quantitative PCR assays on cyclers that require passive reference dye (including high ROX or low ROX reference signal requiring platforms).

Applications

  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination
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