Isolate DNA from gram-positive and negative bacteria using spin columns
The E.Z.N.A.® Bacterial DNA Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of gram-positive and negative bacterial species. This kit uses an optimized lysis condition and up to 1 x 10^9 bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
- Reliable – Reproducible DNA purification from a variety of sample sources
- High yield – Glass beads and enzymatic digestion for cell lysis