HRP-conjugated Goat anti-Rabbit IgG (H+L), 100 μL
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Description
HRP-conjugated Goat anti-Rabbit IgG (H+L), 100 μL
Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Product Name
HRP-conjugated Goat anti-Rabbit IgG (H+L)
Catalog Number
AS014
Isotype
Horseradish peroxidase conjugated IgG
Conjugate
HRP
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Purification Method
Affinity purification
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ABClonal
Based in Massachusetts, ABclonal is a dynamic and growing provider of biology research reagents and services. With scientists from world-class universities, we thrive to improve the quality of life science research by providing high-quality antibodies, proteins, ELISA kits, NGS Prep Kits and molecular enzymes.
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