IQeasy™ plus Plant RNA Extraction Kit
Description
Description
Column spin type product for extracting total RNA from plant specimens such as leaves, seed, root, stem including a leguminous plant
- • Possible to extract total RNA from various plant specimen
- • Total RNA extraction is possible from seed samples containing large amounts of metabolites
– RNA extraction from fauna and legumes
– High purity and high yield of RNA extraction
– Possible to remove gDNA quickly and extract RNA within 20 minutes
– 99% removal of gDNA without DNase
The IQeasy™ Plus Plant RNA Extraction Mini Kit is designed to extract total RNA from tissue samples such as leaves, stems, roots, and seeds of various plant species.In plants, photosynthesis accumulates various metabolites and byproducts such as inhibitors. In order to remove inhibitors, Normally phenol use in the lysis step. However, in case where phenol is not used a high concentration of chaotrophic salt is used for lysis. In such cases, rice, barley, wheat, Beans and seeds (containing large amounts of protein and lipids), such as seeds (containing large amounts of starch) or soybeans, mung beans, peas, etc. In this case, the nucleic acid to be extracted is also precipitated (Co-precipitation) and the RNA content in the final recovered fraction is extremely low. IQeasy™ Plus Plant RNA Extraction Mini Kit is possible to overcome this case.
IQeasy™ Plus Plant RNA Extraction Mini Kit does not use chaotrophic salt during the lysis process to effectively extract total RNA from plant metabolites. Therefore, inhibitor is not eluted with RNA. It is designed to rapidly recover supernatant from homogenate using sieve spin column. It is designed to remove gDNA by applying 2 column system using gDNA binding column to minimize gDNA contamination. As mentioned earlier, there is no enzymatic lysis, therefore, It is possible to extract RNA easily within 20 minutes and it is possible to extract total RNA of high purity and high efficiency with one kit by providing two protocols which are divided into tissue and species.
IKit Contents
| Contents | 50 COLUMNS |
|---|---|
| Buffer RLE | 25ml × 1 ea |
| Buffer RB | 20ml × 1 ea |
| Buffer RLD | 15ml × 1 ea |
| Buffer RPB | 15ml × 1 ea |
| Buffer RW1 | 40ml × 1 ea |
| Buffer RW2 (concentrate) | 10ml × 1 ea |
| Buffer RE | 20ml × 1 ea |
| Sieve Spin Columns | 50 Columns |
| gDNA Remover Spin Columns | 50 Columns |
| Binding Columns (Red O-ring) | 50 Columns |
| Collection Tubes (2.0 ml tubes) | 50 tube |
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