LiliF™ NDV Real-time RT-PCR Kit 50 tests
Description
Background Information
LiliF™ NDV Real-time RT-PCR Kit is able to detect directly and specifically common and patho type of NDV by real-time PCR technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer and probe set for a highly conserved region based on current sequence alignments of all type of NDV allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the real-time RT-PCR (quantitative) method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.
Principle
• This product is a qualitative Real-time PCR testing product with 5’ nuclease assay technology and CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.
• The assay is a real-time RT-PCR that discriminates non pathogenic NDV and pathogenic NDV in one reaction. The assay is composed of two principal steps: (1) nucleic acid extraction f rom specimens, and (2) amplification of the target extracted nucleic acid fragment using fluorescent probe and specific primers pair.
Intended Use
• For Research Use Only, Not for use in diagnostic procedures.
• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.
• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.
Kit Contents
| No. | contents | 50 tests/kit |
|---|---|---|
| 1 | 2X qRT-PCR Master Mix Solution | 280 μl x 2 tubes |
| 2 | NDV Detection Solution | 140 μl x 2 tubes |
| 3 | Positive Control | 25 μl x 3 tubes |
| 4 | DNase/RNase Free Water | 1 ml x 1 tube |
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