MTT, 1g
Description
MTT, or Thiazolyl Blue Tetrazolium Bromide, is a membrane-permeable dye. It is intracellularly reduced by mitochondrial enzymes, leaving a purple color that can then be analyzed to quantify the number of viable cells present in a sample.
MTT is sensitive to light and should be kept in the dark until the assay is complete. MTT concentration can by read by a spectrophotometer at 500-600 nm. The MTT Assay is generally performed at 0.5 mg/well.
MTT is often dissolved in PBS for use in cytotoxicity assays. bioWORLD provides everything necessary for liquid cell culture, including an extensive portfolio of culture media.
Shelf life
4 years
Storage Temperature
2-8°C
ECCN #
EAR99
European Com.#
206-069-5
Hazmat Ship
Non-hazardous
Purity
>98%
Appearance color
Yellow to green-yellow
Appearance form
Powder
Molecular Formula
C18H16BrN5S
Molecular Weight
414.32
Melting point
195°C
Solubility (@ RT)
H2O: 20 mg/mL
Ethanol: 20 mg/mL
2-methoxyethanol: 20 mg/mL
Soluble in PBS.
Pack Size
1g
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