Description

SolisFAST^® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan^® probes), and is suitable for detection and quantitation of up to five targets simultaneously.

Combining our novel in silico- designed SolisFAST^® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice free shipping and reaction set-up.

Applications

• DNA/LNA hydrolysis probe based assays
• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination

Properties

Concentration: 5x

Hot-start: yes, 3 minutes initial incubation at 95 °C

Cycling speed: fast or ultra-fast

Detection type: Probe-based

Reference dye: none

Multiplex: up to 5 plexes simultaneously

Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!

Mix Components:

SolisFAST^® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.

qPCR buffer: Includes 15 mM MgCl₂ (1x PCR solution 3 mM MgCl₂), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR