UltraScence Femto Plus Western Substrate 100mL
Description
The UltraScence Femto Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The UltraScence Femto Plus Western Substrate is designed for the detection of the target proteins in amounts that are too small to be seen with typical ECL substrates. The low femtogram detection (mid-atto) of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
Product Name: UltraScence Femto Plus Western Substrate
Product Item No.: CCH375-B100ML
Size: 100ml
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Contents/Storage: Store at 4℃ for 2 years
US Patent No.: US10,711,185
Figure 2. The membrane images show that UltraScence Femto Plus Western Substrate provide sensitivity the same as Thermo ScientificTM SuperSignalTM West Femto. They can both clearly detect 5 ng EGFP after exposure for 180 sec. Membranes were probed with GFP tag Rabbit PolyAb diluted (1:10,000) and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with 400 μL of UltraScence Femto Plus Western substrate (CCH375-B100ML). The blots were simultaneously exposed for 5, 10, 30, 60 and 180 seconds and using Chemlux SPX-600 Series digital imaging system.
* SuperSignal West Femto is a registered trademark of Thermo Fisher Scientific. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.
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