5x HOT FIREPol® Blend Master Mix Ready to Load 1 ml | 250 rxn
د.إ 375.10
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Description
Ready to load hot-start PCR master mix for more demanding PCR assays
- increased yield, sensitivity and specificity
- up to 5x higher fidelity
- suitable for templates up to 5 kb
- reduced primer dimer formation
- load directly on gel after PCR
- 1 ml | 250 rxn
Mix Components
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
Proofreading enzyme: to enhance fidelity
5x Blend Master Mix Buffer with 7. 5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
Bovine serum albumin (BSA) to enhance PCR reaction
Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment
Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp
Compound that increases sample density for direct loading
SOLIS BIODYNE
Solis BioDyne has been developing and producing life science reagents since 1995, having become one of the leading reagent suppliers in Europe today. High standards for production and service have made Solis BioDyne a trusted trademark worldwide. Solis BioDyne DNA polymerases, PCR Master Mixes, qPCR Mixes and reverse transcription reagents are used by a quickly growing number of customers across the globe, including top research institutes and biotech-companies. Solis BioDyne has partners in both private and state sectors, with cooperation projects ranging from OEM production to scientific research.
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