5x HOT FIREPol® EvaGreen® qPCR Supermix is a laboratory reagent used for real-time quantitative polymerase chain reaction (qPCR) applications. The Supermix is designed for use with EvaGreen® dye, which is a fluorescent DNA-binding dye that is commonly used for real-time PCR detection.
The Supermix is made up of a pre-optimized blend of hot-start Taq DNA polymerase, EvaGreen® dye, dNTPs, buffer, and other reagents required for qPCR amplification. The hot-start Taq DNA polymerase is designed to provide high sensitivity and specificity, while the EvaGreen® dye allows for accurate detection and quantification of amplified DNA in real-time.
Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting
Optimized ready-to-use solution for dye-based real-time quantitative
PCR assays. This master mix has been developed to give highly specific and sensitive results, and demonstrates excellent performance with GC-rich regions.
- high sensitivity with low DNA concentrations
- blue dye to ease pipetting
- reduced primer dimer formation
- one qPCR mix for all cyclers (except capillary)
- contains dUTP to prevent cross-contamination with UNG treatment
- 1 ml | 250 rxn X 5