145 kDa

Polyclonal

Rabbit

This antibody recognizes CD117/c-Kit protein. It cross- reacts to human, mouse and rat. The other species were not tested.

The Rabbit IgG is purified by Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C.
The antibodies can be stored at 2 degree C to 8 degree C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Small volumes of anti-CD117 (kit) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background: The c-Kit proto-oncogene encodes a 145 kDa transmembrane receptor (CD117) with tyrosine kinase activity that is structurally related to platelet-derived growth factor. C-Kit/CD117 antigen is expressed on almost all hematopoietic stem and progenitor cells. c-Kit regulates a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis.

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC), ELISA (EIA), Immunoprecipitation (IP)

Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-10 ug/ml

Optimal dilutions should be determined by researchers for specific applications.