CytoView MEA 24-White plate, 16 microelectrodes per well including one large stimulation electrode per well, white polystyrene walls with a transparent SU-8 bottom, box of 5 plates

Cell visualization and assay multiplexing

The innovative, transparent plate bottom offers additional assay flexibility including cell visualization and assay multiplexing. Bright field imaging enables confirmation of cell spotting accuracy and correlation of cell culture health and connectivity with MEA results. Multiplex fluorescence- or luminescence-based assays with your MEA study to probe complementary end points.

High quality MEA data

Both cardiomyocytes and neurons perform well on the CytoView MEA plates, showing excellent coverage across all wells and the high signal-to-noise ratio Axion customers expect.

Assay multiplexing enriches MEA data

With the transparent CytoView MEA plates, reporter-based (ex. fluorescent or luminescent) assays can be used to complement MEA data generated from the same well. The combination of electrophysiological data with reporter-based assays can provide supplementary information regarding compound mechanism of action.

In this neurotoxicity assay, compound were assessed using MEA electrophysiology data from Axion’s Maestro MEA system multiplexed with a fluorescence lactate dehydrogenase (LDH) cell viability assay. Multiplexing enabled greater specificity for compound classification compared to MEA data alone. (A) Both deltamethrin and tributyltin reduced mean firing rate, but (B) only tributyltin reduced cell viability. (Data provided by external customer)