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Dithiothreitol (DTT, Cleland’s reagent) for biotechnology 5G
د.إ 235.10
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Synonyms: DL-1,4-Dithiothreitol , DTT , (R*,R*)-1,4-Dimercapto-2,3-butandiol
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
| Formula: C₄H₁₀O₂S₂ MW: 154.25 g/mol Boiling Pt: ∼125 °C (1013 hPa) Melting Pt: 42 C…43 °C Flash Pt: >110 °C Storage Temperature: Refrigerator |
MDL Number: MFCD00004877 CAS Number: 3483-12-3 EINECS: 222-468-7 |
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