Description

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol^®, MgCl₂, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.

Ready to Load format also includes a compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis.

We recommend using FIREPol^® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. FIREPol^® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

Properties

Concentration: 5x

Hot-start: no

Ready to load: yes

Fidelity: same as Taq

Cloning Type: T/A Cloning

Amplicon Size: up to 5 kb

Mix Components

FIREPol^® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

12.5 mM MgCl₂: 1x PCR solution – 2.5 mM MgCl₂

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment

Yellow dye: migration equivalent to 35-45 bp DNA fragment

Compound that increases sample density for direct loading