HOT FIREPol® MultiPlex Mix Ready To Load

د.إ 374.10
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04-36-00120

Description

Hot-start master mix for high throughput applications with ready-to-load feature

  • analyze multiple targets per reaction
  • increased sensitivity and yield
  • prevents primer dimer formation
  • reaction set-up and shipment without dry ice
  • 1 ml | 250 rxn

Description

HOT FIREPol® MultiPlex Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers, and water) and an additional compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis.

Generated PCR products are compatible with T/A cloning procedures.

Recommendations

We recommend using HOT FIREPol® MultiPlex Mix Ready To Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.

HOT FIREPol® MultiPlex Mix Ready To Load is not recommended for use in applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Ready to load: yes

Fidelity: 1 x Taq

Cloning Type: T/A cloning

Multiplexing: tested with up to 18 targets (depending on amplicon length)

Amplicon Size: up to 5 kb

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start

5x Multiplex Buffer with 10 mM MgCl21x PCR solution – 2 mM MgCl2

dNTPs: dATP, dCTP, dGTP and dTTP

Bovine serum albumin (BSA) to enhance PCR reaction

Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment

Yellow dye: migration equivalent to 35-45 bp DNA fragment

Compound that increases sample density for direct loading 

SOLIS BIODYNE

Solis BioDyne has been developing and producing life science reagents since 1995, having become one of the leading reagent suppliers in Europe today. High standards for production and service have made Solis BioDyne a trusted trademark worldwide. Solis BioDyne DNA polymerases, PCR Master Mixes, qPCR Mixes and reverse transcription reagents are used by a quickly growing number of customers across the globe, including top research institutes and biotech-companies. Solis BioDyne has partners in both private and state sectors, with cooperation projects ranging from OEM production to scientific research.
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