Hot-start PCR master mix for more demanding PCR assays, 1 ml | 250 rxn
Description
- increased yield, sensitivity and specificity
- up to 5x higher fidelity
- suitable for templates up to 5 kb
- reduced primer dimer formation
Description
HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers, and water).
HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to fivefold) compared to regular Taq polymerase.
In addition, the mix also allows the amplification of longer fragments.
Generated PCR products are compatible with blunt-end and T/A cloning procedures.
Properties
Concentration: 5x
Hot-start: yes, initial activation in 12-15 min
Ready to load: no
Fidelity: 5 x Taq
Cloning Type: T/A cloning and Blunt-end cloning
Amplicon Size:Â up to 5 kb
Mix Components
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
Proofreading enzyme:Â to enhance fidelity
5x Blend Master Mix Buffer with 12. 5 mM MgCl2 1x PCR solution – 2.5 mM MgCl2
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
Bovine serum albumin (BSA)Â to enhance PCR reaction
MgCl2 (mM) final concentration:Â 2.5
SOLIS BIODYNE
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