Pro Ligation-Free Cloning Kit (20 reactions)

د.إ 1,540.00
29 people are viewing this right now
Available on backorder
Free Shipping & Returns:
On all orders over د.إ 400.00
E086

Description

Cat. No. E086
Name Pro Ligation-Free Cloning Kit (20 reactions)
Unit 20 Reactions
Category Molecular Biology Enzymes and Kits
Description The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs.

Key Kit Features:

  • One-step cloning: No more time-consuming, multi-step traditional cloning that depnds on available restrict sites or compatible ends
  • Scar-free cloning: No unwanted/extra base pairs in the inserts
  • Easy-to-customize: Clone in promoter, gene, tag, multi-fragment or whiole vector constructs in one step
  • Ready for downstream applications: Ligase-free reaction, no gel extraction or column purification steps needed
  • Robust assembly: Better performance for difficult, multi-fragment assemblies such as CRISPR multiplex sgRNA vector assembly
  • Increased thermostability: High colony yields

Testimonial:

“I tried the molecular cloning of inserting two DNA fragments (2Kb & 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit.”

– Jianfeng Lan, The Buck Institute

Application
  • Rapid multi-fragment cloning and assembly of whole vector constructs
  • Clone in a promoter, gene, and tag, all in one step
  • Rapid assembly of CRISPR multiplex gRNA vectors in one step
Storage Condition Store at -20°C
Select the fields to be shown. Others will be hidden. Drag and drop to rearrange the order.
  • Image
  • SKU
  • Rating
  • Price
  • Stock
  • Description
  • Weight
  • Dimensions
  • Additional information
  • Attributes
  • Add to cart
Click outside to hide the comparison bar
Compare
0
0
×

Hello!

Click one of our contacts below to chat on WhatsApp

×