SolisFAST® SolisGreen® qPCR Mix , 1mL /250RXN
Description
Product Size
- 1 ml | 250 rxn
Extremely fast and highly sensitive dye-based qPCR
Extremely fast and highly sensitive dye-based qPCR for most demanding applications.
- qPCR results 2x faster
- highly sensitive SolisGreen® dye
- reaction set-up and shipment without dry ice
Properties
Concentration: 5x
Hot-start: yes, 3 minutes initial incubation at 95°C
Cycling speed: fast and ultra-fast
Detection type: dye-based, includes SolisGreen® intercalating dye
Reference dye: based on ROX
Compatible real-time instruments: The mix is compatible with qPCR cyclers where ROX is used as a passive reference signal for normalization of the data. Check Your cycler!
Mix Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
Reference dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel
SolisGreen® dye
This dye-based qPCR mix contains next generation DNA binding dye SolisGreen®. The fluorescence spectra of this dye is similar to the more widely used SYBR® Green I and is compatible with most major realtime cyclers. SolisGreen® dye shows:
- high fluorescence level
- high sensitivity for detecting low template concentrations
- high stability for room temperature storage
SOLIS BIODYNE
There are no question found.
Rating & Review
There are no reviews yet.