SolisFAST® SolisGreen® qPCR Mix , 1mL /250RXN

Description

Product Size

• 1 ml | 250 rxn

Extremely fast and highly sensitive dye-based qPCR

Extremely fast and highly sensitive dye-based qPCR for most demanding applications.

• qPCR results 2x faster
• highly sensitive SolisGreen^® dye
• reaction set-up and shipment without dry ice

Properties

Concentration: 5x

Hot-start: yes, 3 minutes initial incubation at 95°C

Cycling speed: fast and ultra-fast

Detection type: dye-based, includes SolisGreen^® intercalating dye

Reference dye: based on ROX

Compatible real-time instruments: The mix is compatible with qPCR cyclers where ROX is used as a passive reference signal for normalization of the data. Check Your cycler!

Mix Components:

SolisFAST^® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.

qPCR buffer: Includes 7.5 mM MgCl₂ (1X PCR solution 1.5 mM MgCl₂), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction

Reference dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR

Fluorescent dye: SolisGreen^® is a nucleic acid dye detected in the FAM or SYBR^® Green I channel

SolisGreen^® dye

This dye-based qPCR mix contains next generation DNA binding dye  SolisGreen^®. The fluorescence spectra of this dye is similar to the more widely used SYBR^® Green I and is compatible with most major realtime cyclers. SolisGreen^® dye shows:

• high fluorescence level
• high sensitivity for detecting low template concentrations
• high stability for room temperature storage