TEV Protease
Description
| Name | TEV Protease | ||||||||
| Unit | 100 μl | ||||||||
| Category | Molecular Biology Enzymes and Kits | ||||||||
| Description | abm’s TEV Protease is an enhanced version of the site-specific protease derived from Tobacco Etch Virus (TEV). This optimized enzyme offers superior activity, stability, and site-specificity compared to the native form. TEV Protease cleaves fusion proteins with high precision at the Gln-Gly or Gln-Ser bond within the seven-amino acid recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQ(G/S)).
The enzyme is highly effective across a broad range of temperatures (4–30°C, with an optimum at 30°C) and pH values (5.5–9.0), enabling flexible experimental conditions. Under optimal conditions, up to 99% cleavage can be achieved in just 1–2 hours. Additionally, the inclusion of a 6X-His tag at the N-terminus allows for convenient removal of TEV Protease post-cleavage using Ni-IDA Agarose Beads (Cat. No. G250) via affinity chromatography.
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| Application |
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| Concentration | 10U/ul | ||||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E027 |
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