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TEV Protease

د.إ 686.40
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E027
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Description

Name TEV Protease
Unit 100 μl
Category Molecular Biology Enzymes and Kits
Description abm’s TEV Protease is an enhanced version of the site-specific protease derived from Tobacco Etch Virus (TEV). This optimized enzyme offers superior activity, stability, and site-specificity compared to the native form. TEV Protease cleaves fusion proteins with high precision at the Gln-Gly or Gln-Ser bond within the seven-amino acid recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQ(G/S)).

The enzyme is highly effective across a broad range of temperatures (4–30°C, with an optimum at 30°C) and pH values (5.5–9.0), enabling flexible experimental conditions. Under optimal conditions, up to 99% cleavage can be achieved in just 1–2 hours. Additionally, the inclusion of a 6X-His tag at the N-terminus allows for convenient removal of TEV Protease post-cleavage using Ni-IDA Agarose Beads (Cat. No. G250) via affinity chromatography.

Product Component Quantity
TEV Protease (10 U/µl) 100 µl
20X TEV Protease Reaction Buffer 1.0 ml
100 mM DTT 500 µl
Application
  • Cleavage of tags from recombinant fusion proteins containing a TEV recognition site
  • One step affinity removal of His-tagged TEV after cleavage
Concentration 10U/ul
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E027

ABM

Founded in 2004, Applied Biological Materials (abm) Inc. has been catalyzing scientific discoveries in Life Sciences and Drug Development for over a decade. Based in Vancouver, Canada, our agility in developing and commercializing novel research tools and our complete portfolio of unique services have made us an invaluable partner to laboratories and research institutions around the world. Driven by a team of passionate and talented scientists, abm is dedicated to empowering the scientific community with the latest innovations for life sciences research and drug development.
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