Accutase is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including our selection of Advanced Surface coated plasticware. Accutase is useful for cell detachment and for preparing single-cell suspensions from clumped cell populations for sub-culturing cells, analytical studies, and for accurate cell counting.

Accutase is formulated at a concentration that is ready to use, once thawed. (Note: Never thaw Accutase at 37°C.) A thawed bottle of Accutase can be removed from the refrigerator and immediately applied to cells. It should not be pre-warmed to 37°C. Accutase contains proteolytic activity that gently breaks down cellular adhesion molecules and enables cell detachment from the bottom of the flask.

Accutase cell detachment solution is:

• Efficient, with gentle detachment of adherent cells within minutes
• An alternative to Trypsin
• Able to eliminate neutralization or wash steps
• Accutase does not contain mammalian or bacterial derived products