5x HOT FIREPol® EvaGreen® qPCR Supermix 8 ml | 2000 rxn

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Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting

Optimized ready-to-use solution for dye-based real-time quantitative
PCR assays. This master mix has been developed to give highly specific and sensitive results, and demonstrates excellent performance with GC-rich regions.

  • high sensitivity with low DNA concentrations
  • blue dye to ease pipetting
  • reduced primer dimer formation
  • one qPCR mix for all cyclers (except capillary)
  • contains dUTP to prevent cross-contamination with UNG treatment
  • 8 ml | 2000 rxn


HOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments.

The Mix contains HOT FIREPol® DNA Polymerase and a passive internal reference dye that is compatible with most qPCR cyclers including no ROX and low ROX* reference signal requiring platforms.

The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with real-time detection.


  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination


Concentration: 5x

Hot-start: yes, initial activation in 12 min

Detection type: dye-based, includes EvaGreen® intercalating dye

Reference dye*: based on ROX

Compatible real-time instruments: most qPCR cyclers. Check Your cycler!

IMPORTANT UPDATE! HOT FIREPol® EvaGreen® qPCR Supermix is not compatible with high ROX cyclers such as Applied BioSystems® StepOne™ or StepOnePlus™

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start

5x EvaGreen® qPCR buffer with 12. 5 mM MgCl21x PCR solution – 2.5 mM MgCl2

dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
IMPORTANT: UNG is not included in the HOT FIREPol® EvaGreen® Supermix

EvaGreen® dye

Internal reference based on ROX dye


Blue visualisation dye

EvaGreen Dye

EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is an extremely stable dye, and has been shown to be non-mutagenic and non-cytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!

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