5x HOT FIREPol® SolisGreen qPCR Mix

د.إ 426.10
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08-46-00001

Description

Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets.

Optimized ready-to-use solution for dye-based real-time quantitative
PCR assays. SolisGreen® dye is characterized by high sensitivity and
great PCR efficiency with low template amounts for accurate and
reproducible results

  • improved detection of low target concentrations
  • higher fluorescence level
  • reaction set-up and shipment without dry ice
  • 1 ml | 250 rxn

Description

Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR.

HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye.

It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers.

HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications

  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination
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