Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets.
Optimized ready-to-use solution for dye-based real-time quantitative
PCR assays. SolisGreen® dye is characterized by high sensitivity and
great PCR efficiency with low template amounts for accurate and
- improved detection of low target concentrations
- higher fluorescence level
- reaction set-up and shipment without dry ice
- 1 ml | 250 rxn
Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR.
HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye.
It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination