Zymo DNase I Set, 250 U
د.إ 663.10
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Description
HIGHLIGHTS
- Superior activity.
- Certified RNase-free and protease-free.
- Withstand more than 10 freeze-thaw cycles.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Unit Definition | One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz. |
|---|---|
| Storage | After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C). |
| Reconstitution | Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators. |
| Inactivation | Heat inactivate at 75 °C for 10 min with 5 mM EDTA. |
- Remove DNA from protein and RNA samples.
- Preparation of DNA-free RNA.
- DNA labeling by nick-translation.
- Removal of DNA template after in vitro transcription.
- Removal of DNA from RNA samples prior to downstream applications (NextGen sequencing, RT-PCR).
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