- Superior activity.
- Certified RNase-free and protease-free.
- Withstand more than 10 freeze-thaw cycles.
DNase I is an endonuclease that nonspecifically cleaves single- and double-stranded DNA. It requires divalent metal cations to be active.
|Unit Definition||One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.|
|Storage||After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).|
|Reconstitution||Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.|
|Inactivation||Heat inactivate at 75 °C for 10 min with 5 mM EDTA.|
- Remove DNA from protein and RNA samples.
- Preparation of DNA-free RNA.
- DNA labeling by nick-translation.
- Removal of DNA template after in vitro transcription.
- Removal of DNA from RNA samples prior to downstream applications (NextGen sequencing, RT-PCR).