Zymo DNase I Set, 250 U

د.إ 620.00
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  • Superior activity.
  • Certified RNase-free and protease-free.
  • Withstand more than 10 freeze-thaw cycles.

DNase I is an endonuclease that nonspecifically cleaves single- and double-stranded DNA. It requires divalent metal cations to be active.




Unit Definition One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
Storage After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
Reconstitution Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
Inactivation Heat inactivate at 75 °C for 10 min with 5 mM EDTA.
  • Remove DNA from protein and RNA samples.
  • Preparation of DNA-free RNA.
  • DNA labeling by nick-translation.
  • Removal of DNA template after in vitro transcription.
  • Removal of DNA from RNA samples prior to downstream applications (NextGen sequencing, RT-PCR).

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