Isolate total RNA from plant samples using spin columns
E.Z.N.A.® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogenizer column for filtration and homogenization of viscous plant cell lysate by centrifugation in combination with the HiBind RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, Northern blot analysis, differential display, and poly A RNA selection.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||50-100 μL|
|Starting Material||Plant tissue|
|Starting Amount||<100 mg|
|RNA Yield||Up to 100 µg, >200 nt|
|Processing Mode||Spin column|
|Throughput||1 – 24|
|RNA Binding Technology||Silica technology|
|Binding Capacity||100 µg|