HincII 1.000 Units
Description
For in vitro use only!
Unit Definition: One unit is the amount of enzyme required to completely digest 1 μg of Lambda DNA in 1 hour in a total reaction volume of 50 μl. Enzyme activity was determined in the recommended reaction buffer.
Shipping:Â shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life:Â 12 months
Form: liquid (Supplied in 10 mM Tris-HCl pH 7.4 at 4°C, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% [v/v] Triton™X-100, 5 μg/ml PLL, 500 μg/ml BSA and 50 % [v/v] glycerol)
Source: Haemophilus influenzae Rc
Supplied with:Â 10x Universal Buffer (UB)
Recommended 50 μl assay
| 5 μl | 10x Universal Buffer (UB) |
| 1 μg | pure DNA1 or PCR product2 |
| 10 units | enzyme |
| fill up to 50 μl | PCR grade water |
Reaction Buffer Compatibility:
Our restriction enzymes are fully compatible to restrictases and buffer systems from other manufacturers and can be used along in double digestions. To obtain best results, consult the corresponding manuals of all involved products.
DNA Methylation:
No Inhibition: dam, dcm
Potential Inhibition: EcoKI, CpG
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