HOT FIREPol® EvaGreen® qPCR Supermix

د.إ 5,371.00
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08-36-00001-10

Description

Optimized ready-to-use solution for dye-based real-time quantitative
PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions.

  • high sensitivity with low DNA concentrations
  • blue dye to ease pipetting
  • reduced primer dimer formation
  • one qPCR mix for all cyclers (except capillary and high ROX* cyclers)
  • contains dUTP to prevent carryover contamination with UNG treatment
  • 10 x 1ml | 2500 rxn

Applications

  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start

5x EvaGreen® qPCR buffer with 12. 5 mM MgCl21x PCR solution – 2.5 mM MgCl2

dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
IMPORTANT: UNG is not included in the HOT FIREPol® EvaGreen® Supermix

EvaGreen® dye

Internal reference based on ROX dye

GC-enhancer

Blue visualisation dye

 

SOLIS BIODYNE

Solis BioDyne has been developing and producing life science reagents since 1995, having become one of the leading reagent suppliers in Europe today. High standards for production and service have made Solis BioDyne a trusted trademark worldwide. Solis BioDyne DNA polymerases, PCR Master Mixes, qPCR Mixes and reverse transcription reagents are used by a quickly growing number of customers across the globe, including top research institutes and biotech-companies. Solis BioDyne has partners in both private and state sectors, with cooperation projects ranging from OEM production to scientific research.
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