[KD Validated] Vimentin Rabbit pAb 100 μL

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A11952
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[KD Validated] Vimentin Rabbit pAb 100 μL

Western blot analysis of various lysates using [KD Validated] Vimentin Rabbit pAb (A11952) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
  • WB1:500 – 1:1000
  • IHC-P1:50 – 1:200
  • IF/ICC1:50 – 1:200
  • ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

ABClonal

Based in Massachusetts, ABclonal is a dynamic and growing provider of biology research reagents and services. With scientists from world-class universities, we thrive to improve the quality of life science research by providing high-quality antibodies, proteins, ELISA kits, NGS Prep Kits and molecular enzymes.
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