Live and Dead Cell Staining Kit

Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Small volumes of Live and Dead Cell staining kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Principle of the Assay: Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Distinguishing between live and dead cells is very important for investigation of growth cont rol and cell death. The Live/Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: pla sma membrane integrity and intracellular esterase activity. The kit utilizes LiveDye, a cell cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and NucleiDye, a cell non non-permeable red fluorescent dye