MboII 250 Units
Description
Unit Definition: One unit is the amount of enzyme required to completely digest 1 μg of unmethylated Lambda DNA in 1 hour. Total reaction volume is 50 μl. Enzyme activity was determined in the recommended reaction buffer.
Shipping:Â shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life:Â 12 months
Form: liquid (Supplied in 10 mM Tris-HCl pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 100 μg/ml BSA, 0.1 % Triton X-100 and 50 % [v/v] glycerol)
Source:Â Moraxella bovis
Supplied with:Â 10x Universal Buffer (UB)
Recommended 50 μl assay
| 5 μl | 10x Universal Buffer (UB) |
| 1 μg | pure DNA1 or PCR product2 |
| 10 units | enzyme |
| fill up to 50 μl | PCR grade water |
Reaction Buffer Compatibility:
Our restriction enzymes are fully compatible to restrictases and buffer systems from other manufacturers and can be used along in double digestions. To obtain best results, consult the corresponding manuals of all involved products.
DNA Methylation:
No Inhibition: dcm, EcoKI
Potential Inhibition: dam, CpG
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