Miracle-Star™ Western Blot Detection System 200ml
Description
Description
High intensity, fast reaction, high sensitivity Chemiluminescence Western Blot products
- • Able to detect pico-grams(pg) level of antigen on membrane blot.
- • High Signal Intensity – Generate high contrast signals
- • Duration Time – Maintain stable luminescence for 2 hours after reaction
- • High reactivity – Excellent chemiluminescence reaction can be obtained by incubation for 1 to 5 min
- • Economical – Highly reactive (with HRP) products can reduce the use of secondary antibodies
Miracle-Star™ Western Blot Detection System is able to detect specific antigen(protein) by chemiluminescence method using HRP(Horse radish peroxidase) with powerful signal intensity and high sensitivity. Western Blot Detection is common and essential method for protein expression research. The procedure is as follows. First, implement electrophoresis proteins including specific antigen by SDS-PAGE and then transfer to membrane like PVD and NC. After that, block the proteins that are not binding with membrane by BSA, skim milk or Gelatin solution and specific antigen(protein) binds with specific antibody. The Fc region of antibody binds with secondary antibody. At the secondary antibody, AP (Alkaline phosphatase) or HRP (Horse radish peroxidase) is binding.
Applications
- 01Western Blotting using chemiluminescence
- 02Any Blotting methods (Southern, Northern) which use biotin-avidin-HRP
Kit Contents
| Contents | 16028 |
|---|---|
| Substrate Solution | 100ml × 1 ea |
| Enhancer Solution | 100ml × 1 ea |
| Manual | 1 ea |
Intron Biological
There are no question found.
Rating & Review
There are no reviews yet.