SOLIScript® Fast 1-step RT-qPCR Mix with UNG

Description

SOLIScript^® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG™ Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.

Applications

• Detection and quantification of RNA and DNA targets
• DNA/LNA hydrolysis probe-based assays
• Suitable for singleplex and multiplex reactions (up to 5 plex)

Properties

Sample type: RNA and DNA
Product format: Reagent kit (nuclease-free water supplied in a separate tube)
Detection type: Probe-based
Reference dye: none
Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, switch off ROX normalization prior to the run. Check your cycler!*

*Version with ROX available upon request.

Reagents

SOLIScript^® Fast 1-step RT-qPCR Mix with UNG comprises:

• 4x SOLIScript^® Fast 1-step RT-qPCR Mix with UNG (SOLIScript^® Reverse Transcriptase, RiboGrip^TM RNase Inhibitor, HOT SolisFAST^® DNA polymerase, Salini UNG^TM Uracil-N-Glycosylase, a unique reaction buffer with dNTPs (including dUTPs), 22.0 mM MgCl₂ (1x RT-qPCR solution – 5.5 mM MgCl₂) and additives to maximize PCR efficiency).
• Water, nuclease-free