SOLIScript® RT cDNA synthesis KIT

Description

SOLIScript^® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.

SOLIScript^® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).

SOLIScript^® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.

Properties

Sample type: RNA

Final product: cDNA (first strand)

Product format: Enzyme with all components included reagent kit

Enzyme: SOLIScript^® Reverse Transcriptase

Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript^® Reverse Transcriptase gene.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.