TBE Buffer, or Tris-Borate-EDTA, is a popular choice for gel electrophoresis. It is chosen over TAE Buffers for nucleic acid fragments smaller than 1500 bp. TBE is an ideal buffer for longer and higher voltage electrophoresis.
TBE Buffer 10X is prepared using high quality reagents in triple-distilled, deionized water. The 10X concentration can easily be diluted to a 1X working concentration containing 89mM Tris, 89mM Borate, and 2mM EDTA.