Cetrimide Agar Plate

Description

INTENDED USE: For the isolation and enumeration of Pseudomonas species in water, food products, and environmental samples.

PRINCIPLE:
Pseudomonas spp. are aerobic, rod-shaped bacteria that do not form spores. These Gram-negative organisms are commonly found in water, soil, vegetation, and humid environments. Pseudomonas aeruginosa is particularly versatile, able to grow in distilled water, survive in disinfectants, contaminate cosmetics, and persist in food products. Pseudomonas Selective Agar is specifically designed for isolating and identifying P. aeruginosa. This medium builds on the work of King, Ward, and Raney in 1954, who developed formulations to enhance the production of key pigments, pyocyanin and fluorescein. The addition of cetrimide, refined by Lowbury and Collins, helps inhibit other microorganisms, ensuring the selective growth of Pseudomonas. This agar meets international standards, including those set by the European, United States, and Japanese Pharmacopeias, as well as ISO 22717 and FDA-BAM, making it suitable for detecting P. aeruginosa in cosmetics, non-sterile pharmaceuticals, water, and food samples. Key ingredients include: – Pancreatic digest of gelatin: Provides essential nutrients for bacterial growth and pigment production. – Cetrimide: A detergent that disrupts bacterial membranes, effectively inhibiting non-*Pseudomonas* species. – Magnesium chloride and potassium sulfate: Stimulate the production of the pigments fluorescein and pyocyanin. – Glycerol: Acts as a carbon source and promotes pigment synthesis. This medium allows easy identification of P. aeruginosa based on its characteristic pigment production and colony appearance, making it a dependable choice for microbiological testing across various industries.

STORAGE CONDITIONS AND SHELF LIFE:
• Upon receipt, store at +2 – 8°C away from direct light in a cool, dry place. The user is responsible for the storage method (temperature) of the medium.
• If properly stored, the product may be used up to the expiration date. Do not use it beyond the mentioned expired date.