E.Z.N.A.® Cycle Pure Kit | PCR Clean Up Kit
The E.Z.N.A.® Cycle-Pure Kit is designed for the rapid purification of single or double-stranded DNA from PCR and other enzymatic reactions. The system follows a “bind-wash-elute” procedure and completely removes primers, nucleotides enzymes, salts, and other impurities from a DNA sample. This convenient spin-column format eliminates the need for expensive resins or toxic organic compounds such as phenol and chloroform, thereby making it possible to process multiple samples in parallel. Purified DNA can be used in T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions.
- Rapid – Purification of PCR products in less than 10 minutes
- Safe – No Phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for a variety of downstream applications
For Research Use Only. Not for use in diagnostic procedures.
|Downstream application||T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions|
|Elution volume||30-50 µL|
|Starting material||ssDNA, dsDNA, PCR products|
|Starting amount||5-50 µL|
|DNA recovered||>90% recovery, 100 bp to 10 kb|
|Processing mode||Manual (centrifugation or vacuum)|
|DNA binding technology||Silica mini spin column|
|Processing time||<10 minutes|
|Special Notes||For clean-up of DNA fragments < 200 bp, please follow the protocol modification outlined in the product manual|