Isolates RNA from small samples with lower elution volumes using spin columns
E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as laser dissected samples (LDS) or fine-needle aspirates (FNA). Normally, up to 5 x 105 eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows the processing of single or multiple samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10-15 µL nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, poly A purification, nuclease protection and in vitro translation.
For Research Use Only. Not for use in diagnostic procedures.
|Downstream Application||PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification|
|Elution Volume||15-20 μL|
|Starting Material||Cultured eukaryotic cells and soft tissues|
|Starting Amount||Up to 5 x 105 eukaryotic cells or 5 mg tissue|
|RNA Yield||Up to 50 µg|
|Processing Mode||Manual, centrifugation|
|Throughput||1 – 24|
|RNA Binding Technology||Silica Micro spin column|
|Binding Capacity||50 µg|
Protocol and Resources
Product Documentation & Literature
RNA yield for E.Z.N.A.® MicroElute Total RNA Kit.
Figure 1. Yield of total RNA purified using the E.Z.N.A.® MicroElute Total RNA Kit. Purified total RNA from 16 samples of 0.5 mg of fish liver was isolated with the E.Z.N.A.® MicroElute Total RNA Kit. RNA was eluted in 20 µL DEPC-treated water and quantitated using Thermo Fisher’s NanoDrop® 2000.
RNA was further analyzed using a gel electrophresis.
Figure 2. Total RNA purified using the E.Z.N.A.® MicroElute Total RNA Kit. Purified total RNA from 0.2 mg chicken liver was isolated with the E.Z.N.A.® MicroElute Total RNA Kit. Total RNA (100 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.